Journal: The FASEB Journal

Article Title: Interaction between ferroptosis and TNF‐α: Impact in obesity‐related osteoporosis

doi: 10.1096/fj.202201958r

Figure Lengend Snippet: FIGURE 4 TNF-α modulates susceptibility to ferroptosis in MG63 and HUVEC. (A) The TNFR2 /TNFR1 mRNA expression ratio determined by qRT-PCR analysis in MG63 and HUVEC at 72 h after exposure to TNF-α. (B) Detection and quantitative analysis of intracellular ROS production in MG63 and HUVEC upon 72 h TNF-α treatment using DCFH-DA probes. Scale bar: 50 μm. (C,D) Relative mRNA expression of TNFR2 in MG63 and HUVEC transfected with scrambled siRNA (snc) or siRNA (siRNA #1 and siRNA#2) targeting TNFR2. (E,F) Western blotting analysis of SLC7A11,GCLC and GCLM in MG63 and HUVEC transfected with scrambled siRNA (snc) and siRNA#1 targeting TNFR2, and subsequently treated with TNF-α for 72 h. (G,H) Relative cell viability of MG63 and HUVEC primed with TNF-α for 72 h, followed by different concentrations of erastin treatment. Data are shown as mean ± SD representative of three independent experiments. *p < .05; **p < .01; ***p < .001.

Article Snippet: Human osteoblast- like cell line MG63 and human umbilical vein endothelial cell HUVEC were purchased from American Type Culture Collection (Rockville, MD, USA).

Techniques: Expressing, Quantitative RT-PCR, Transfection, Western Blot

Journal: The FASEB Journal

Article Title: Interaction between ferroptosis and TNF‐α: Impact in obesity‐related osteoporosis

doi: 10.1096/fj.202201958r

Figure Lengend Snippet: FIGURE 5 TNF-α rescues osteogenic and angiogenic dysfunctions caused by low-dose erastin through ferroptosis inhibition. After 7 days of osteogenic differentiation, (A,B) Relative mRNA expression of RUNX2 and COL1A1. (C–E) Western blotting analysis of RUNX2 and COL1A1 in MG63 treated with vehicle (DMSO), erastin, or both erastin and TNF-α. (F–H) Images and quantification analysis of capillary-like structures formed by HUVECs treated with vehicle (DMSO), erastin, or both erastin and TNF-α in the tube formation assay. Scale bar:100 μm. Data are shown as mean ± SD representative of three independent experiments. *p < .05; **p < .01; ***p < .001.

Article Snippet: Human osteoblast- like cell line MG63 and human umbilical vein endothelial cell HUVEC were purchased from American Type Culture Collection (Rockville, MD, USA).

Techniques: Inhibition, Expressing, Western Blot, Tube Formation Assay

Journal: The FASEB Journal

Article Title: Interaction between ferroptosis and TNF‐α: Impact in obesity‐related osteoporosis

doi: 10.1096/fj.202201958r

Figure Lengend Snippet: FIGURE 6 Ferroptosis inhibitors suppress the cytotoxicity of TNF-α on MG63 and HUVEC. (A,B) Intracellular ROS levels in MG63 and HUVEC treated with vehicle (DMSO), TNF-α, TNF-α+ ferrostatin-1 (Fer-1), or TNF-α+ deferoxamine (DFO). After 7 days of osteogenic induction, (C) relative mRNA expression of ALP (D) Representative images of ALP staining, (E) Western blotting analysis of RUNX2 and COL1A1 in MG63 treated with vehicle (DMSO), TNF-α, TNF-α+ ferrostatin-1 (Fer-1), or TNF-α+ deferoxamine (DFO). (F–H) Images and quantification analysis of capillary-like structures formed by HUVECs treated with vehicle (DMSO), TNF-α, TNF-α +ferrostatin-1 (Fer-1), or TNF-α+ deferoxamine (DFO). Data are shown as mean ± SD representative of three independent experiments. *p < .05; **p < .01; ***p < .001.

Article Snippet: Human osteoblast- like cell line MG63 and human umbilical vein endothelial cell HUVEC were purchased from American Type Culture Collection (Rockville, MD, USA).

Techniques: Expressing, Staining, Western Blot

Journal: The FASEB Journal

Article Title: Interaction between ferroptosis and TNF‐α: Impact in obesity‐related osteoporosis

doi: 10.1096/fj.202201958r

Figure Lengend Snippet: FIGURE 7 Schematic diagram illustrating the interaction between TNF-α and ferroptosis in MG63 and HUVEC.

Article Snippet: Human osteoblast- like cell line MG63 and human umbilical vein endothelial cell HUVEC were purchased from American Type Culture Collection (Rockville, MD, USA).

Techniques:

Journal: Advanced Materials Technologies

Article Title: Influence of Initial Surface Roughness on LIPSS Formation and Its Consecutive Impact on Cell/Bacteria Attachment for TiAl6V4 Surfaces

doi: 10.1002/admt.202201802

Figure Lengend Snippet: Figure 7. Behavior of MG63 osteoblastic cells on unpolished and polished laser modified TiAl6V4 alloy. A) Cell viability, B) alkaline phosphatase activity, and C) low magnification SEM representative images. *Significantly different from the respective unlasered control, p ⩽0.05.

Article Snippet: Human osteoblast-like MG63 cell line (ATCC CRL14271) was used for in vitro cytocompatibility assessment.

Techniques: Activity Assay, Control